JOVE JOURNAL

Genetics

Showing 109 - 120 in 413 videos.

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Journal (Genetics)

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Yu Wang¹, Andrew J. H. Smith¹, David C. Hay¹

¹MRC Centre for Regenerative Medicine,University of Edinburgh

Journal (Genetics)

Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells

Hotake Takizawa¹, Mitsuto Sato¹, Yoshitsugu Aoki¹

¹Department of Molecular Therapy, National Institute of Neuroscience,National Center of Neurology and Psychiatry

Journal (Genetics)

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)

Tian V. Tian^1,2,3, Enrique Vidal^1,2, Thomas Graf^1,2, Grégoire Stik^1,2

¹Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, ²Universitat Pompeu Fabra, ³Vall d'Hebron Institute of Oncology

Journal (Genetics)

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Hirofumi Nishizono*^1,2,3, Mohamed Darwish*^4,5, Hideki Uosaki^6,7, Nanami Masuyama^8,9,10, Motoaki Seki^8,11, Hiroyuki Abe³, Nozomu Yachie^8,9,10,12,13, Ryohei Yasuda¹

¹Max Planck Florida Institute for Neuroscience, ²Life Science Research Center,University of Toyama, ³Department of Biochemical Engineering, Graduate School of Science and Engineering,Yamagata University, ⁴Graduate School of Innovative Life Science,University of Toyama, ⁵Department of Biochemistry, Faculty of Pharmacy,Cairo University, ⁶Division of Regenerative Medicine, Center for Molecular Medicine,Jichi Medical University, ⁷Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology,Jichi Medical University, ⁸Synthetic Biology Division, Research Center for Advanced Science and Technology,University of Tokyo, ⁹Institute for Advanced Biosciences,Keio University, ¹⁰Graduate School of Media and Governance,Keio University, ¹¹Department of Molecular Oncology, Graduate School of Medicine,Chiba University, ¹²Department of Biological Sciences, School of Science,University of Tokyo, ¹³College of Arts and Sciences,University of Tokyo

Journal (Genetics)

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Krzysztof J. Skowronek¹, Matthias Bochtler¹

¹International Institute of Molecular and Cell Biology, Warsaw (IIMCB)

Journal (Genetics)

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Cheryl A. Hobbs*¹, Leslie Recio¹, John Winters¹, Kristine L. Witt²

¹Toxicology Program, ILS, Inc., ²Division of the National Toxicology Program,National Institute of Environmental Health Sciences

Journal (Genetics)

A Screening Method for Identification of Heterochromatin-Promoting Drugs Using Drosophila

Lin Zhang¹, Kenny Dao¹, Angela Kang¹, Andre C. Loyola¹, Robin Shang¹, Jinghong Li¹, Willis X. Li¹

¹Department of Medicine,University of California San Diego

Journal (Genetics)

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Bas B. Oude Munnink¹, David F. Nieuwenhuijse¹, Reina S. Sikkema¹, Marion Koopmans¹

¹ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

Journal (Genetics)

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Yaolei Mi^1,2, Zhihui Zhu³, Guangtao Qian³, Yu Li⁴, Xiangxiao Meng¹, Jianping Xue³, Qingfu Chen⁵, Wei Sun¹, Yuhua Shi^1,2

¹Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences, ²Artemisinin Research Center,China Academy of Chinese Medical Sciences, ³College of Life Science,Huaibei Normal University, ⁴Economic Crop Research Institute Sichuan Academy of Agriculture Sciences, ⁵Research Center of Buckwheat Industry Technology,Guizhou Normal University

Journal (Genetics)

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Rafael J. L. Morcillo¹, Achen Zhao^1,2, María I. Tamayo-Navarrete³, José M. García-Garrido³, Alberto P. Macho¹

¹Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences, ²University of Chinese Academy of Sciences, ³Department of Soil Microbiology and Symbiotic Systems,Estación Experimental del Zaidín (CSIC)

Journal (Genetics)

Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence

Sean J. O'Sullivan^1,2, Beverly A.S. Reyes³, Rajanikanth Vadigepalli¹, Elisabeth J. Van Bockstaele³, James S. Schwaber¹

¹Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology,Thomas Jefferson University, ²Sidney Kimmel Medical College,Thomas Jefferson University, ³Department of Pharmacology & Physiology,Drexel University College of Medicine

Journal (Genetics)

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Francis Migneault^1,2,3, Frédéric Gagnon^2,3, Emmanuelle Brochiero^1,3, Yves Berthiaume^2,3, André Dagenais^2,3

¹Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), ²Institut de Recherches Cliniques de Montréal (IRCM), ³Département de médecine, Faculté de médecine,Université de Montréal

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