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JOVE JOURNAL
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Journal (Genetics)
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
Yelena Levin*
1
,
Keyur Talsania*
1,2
,
Bao Tran
1
,
Jyoti Shetty
1
,
Yongmei Zhao
1,2
,
Monika Mehta
1
1
NCI CCR Sequencing Facility
,
Frederick National Laboratory for Cancer Research
,
2
Advanced Biomedical and Computational Sciences
,
Frederick National Laboratory for Cancer Research
Author
Yelena Levin*
1
,
Keyur Talsania*
1,2
,
Bao Tran
1
,
Jyoti Shetty
1
,
Yongmei Zhao
1,2
,
Monika Mehta
1
Institution
1
NCI CCR Sequencing Facility
,
Frederick National Laboratory for Cancer Research
,
2
Advanced Biomedical and Computational Sciences
,
Frederick National Laboratory for Cancer Research
AUTHORS
Journal (Genetics)
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
Sumana Sharma
1,2
,
Gavin J. Wright
1
1
Cell Surface Signalling Laboratory
,
Wellcome Trust Sanger Institute
,
2
EMBL-EBI
,
Wellcome Genome Campus
Author
Sumana Sharma
1,2
,
Gavin J. Wright
1
Institution
1
Cell Surface Signalling Laboratory
,
Wellcome Trust Sanger Institute
,
2
EMBL-EBI
,
Wellcome Genome Campus
AUTHORS
Journal (Genetics)
High-Throughput Quantitative RT-PCR in Single and Bulk
C. elegans
Samples Using Nanofluidic Technology
Laetitia Chauve*
1
,
Jérémie Le Pen*
2,3,5
,
Francesca Hodge
1
,
Pia Todtenhaupt
1
,
Laura Biggins
1
,
Eric A. Miska
2,3,4
,
Simon Andrews
1
,
Olivia Casanueva
1
1
Babraham Institute
,
2
Gurdon Institute
,
University of Cambridge
,
3
Department of Genetics
,
University of Cambridge
,
4
Wellcome Trust Genome Campus
,
Wellcome Trust Sanger Institute
,
5
Laboratory of Virology and Infectious Disease
,
The Rockefeller University
Author
Laetitia Chauve*
1
,
Jérémie Le Pen*
2,3,5
,
Francesca Hodge
1
,
Pia Todtenhaupt
1
,
Laura Biggins
1
,
Eric A. Miska
2,3,4
,
Simon Andrews
1
,
Olivia Casanueva
1
Institution
1
Babraham Institute
,
2
Gurdon Institute
,
University of Cambridge
,
3
Department of Genetics
,
University of Cambridge
,
4
Wellcome Trust Genome Campus
,
Wellcome Trust Sanger Institute
,
5
Laboratory of Virology and Infectious Disease
,
The Rockefeller University
AUTHORS
Journal (Genetics)
Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in
Culex quinquefasciatus
Michelle Bui
1
,
Ming Li
1
,
Robyn R Raban
1
,
Nannan Liu
2
,
Omar S Akbari
1,3
1
Section of Cell and Developmental Biology
,
University of California, San Diego
,
2
Department of Entomology and Plant Patholog
,
Auburn University
,
3
Tata Institute for Genetics and Society
,
University of California, San Diego
Author
Michelle Bui
1
,
Ming Li
1
,
Robyn R Raban
1
,
Nannan Liu
2
,
Omar S Akbari
1,3
Institution
1
Section of Cell and Developmental Biology
,
University of California, San Diego
,
2
Department of Entomology and Plant Patholog
,
Auburn University
,
3
Tata Institute for Genetics and Society
,
University of California, San Diego
AUTHORS
Journal (Genetics)
Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
Xianju Bi
1,2
,
Xuechun Zhang
1
,
Xiaohua Shen
1
1
Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences
,
Tsinghua University
,
2
Department of Molecular Biology
,
University of Texas Southwestern Medical Center
Author
Xianju Bi
1,2
,
Xuechun Zhang
1
,
Xiaohua Shen
1
Institution
1
Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences
,
Tsinghua University
,
2
Department of Molecular Biology
,
University of Texas Southwestern Medical Center
AUTHORS
Journal (Genetics)
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
Jos G.A. Smits*
1
,
Jieqiong Qu*
1
,
Hanna Niehues
2
,
Huiqing Zhou
1,3
1
Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences
,
Radboud University, Nijmegen, The Netherlands
,
2
Department of Dermatology, Radboud University Medical Center
,
Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands
,
3
Department of Human Genetics
,
Radboud University Medical Center, Nijmegen, The Netherlands
Author
Jos G.A. Smits*
1
,
Jieqiong Qu*
1
,
Hanna Niehues
2
,
Huiqing Zhou
1,3
Institution
1
Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences
,
Radboud University, Nijmegen, The Netherlands
,
2
Department of Dermatology, Radboud University Medical Center
,
Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands
,
3
Department of Human Genetics
,
Radboud University Medical Center, Nijmegen, The Netherlands
AUTHORS
Journal (Genetics)
Methylated RNA Immunoprecipitation Assay to Study m
5
C Modification in Arabidopsis
Eleftheria Saplaoura*
1
,
Valentina Perrera*
2
,
Vincent Colot
3
,
Friedrich Kragler
1
1
Max Planck Institute of Molecular Plant Physiology
,
2
Department of Molecular Medicine, Medical School
,
University of Padua
,
3
Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France
Author
Eleftheria Saplaoura*
1
,
Valentina Perrera*
2
,
Vincent Colot
3
,
Friedrich Kragler
1
Institution
1
Max Planck Institute of Molecular Plant Physiology
,
2
Department of Molecular Medicine, Medical School
,
University of Padua
,
3
Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France
AUTHORS
Journal (Genetics)
Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
Yu Wang
1
,
Andrew J. H. Smith
1
,
David C. Hay
1
1
MRC Centre for Regenerative Medicine
,
University of Edinburgh
Author
Yu Wang
1
,
Andrew J. H. Smith
1
,
David C. Hay
1
Institution
1
MRC Centre for Regenerative Medicine
,
University of Edinburgh
AUTHORS
Journal (Genetics)
Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells
Hotake Takizawa
1
,
Mitsuto Sato
1
,
Yoshitsugu Aoki
1
1
Department of Molecular Therapy, National Institute of Neuroscience
,
National Center of Neurology and Psychiatry
Author
Hotake Takizawa
1
,
Mitsuto Sato
1
,
Yoshitsugu Aoki
1
Institution
1
Department of Molecular Therapy, National Institute of Neuroscience
,
National Center of Neurology and Psychiatry
AUTHORS
Journal (Genetics)
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)
Tian V. Tian
1,2,3
,
Enrique Vidal
1,2
,
Thomas Graf
1,2
,
Grégoire Stik
1,2
1
Center for Genomic Regulation (CRG)
,
The Barcelona Institute of Science and Technology
,
2
Universitat Pompeu Fabra
,
3
Vall d'Hebron Institute of Oncology
Author
Tian V. Tian
1,2,3
,
Enrique Vidal
1,2
,
Thomas Graf
1,2
,
Grégoire Stik
1,2
Institution
1
Center for Genomic Regulation (CRG)
,
The Barcelona Institute of Science and Technology
,
2
Universitat Pompeu Fabra
,
3
Vall d'Hebron Institute of Oncology
AUTHORS
Journal (Genetics)
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
Hirofumi Nishizono*
1,2,3
,
Mohamed Darwish*
4,5
,
Hideki Uosaki
6,7
,
Nanami Masuyama
8,9,10
,
Motoaki Seki
8,11
,
Hiroyuki Abe
3
,
Nozomu Yachie
8,9,10,12,13
,
Ryohei Yasuda
1
1
Max Planck Florida Institute for Neuroscience
,
2
Life Science Research Center
,
University of Toyama
,
3
Department of Biochemical Engineering, Graduate School of Science and Engineering
,
Yamagata University
,
4
Graduate School of Innovative Life Science
,
University of Toyama
,
5
Department of Biochemistry, Faculty of Pharmacy
,
Cairo University
,
6
Division of Regenerative Medicine, Center for Molecular Medicine
,
Jichi Medical University
,
7
Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology
,
Jichi Medical University
,
8
Synthetic Biology Division, Research Center for Advanced Science and Technology
,
University of Tokyo
,
9
Institute for Advanced Biosciences
,
Keio University
,
10
Graduate School of Media and Governance
,
Keio University
,
11
Department of Molecular Oncology, Graduate School of Medicine
,
Chiba University
,
12
Department of Biological Sciences, School of Science
,
University of Tokyo
,
13
College of Arts and Sciences
,
University of Tokyo
Author
Hirofumi Nishizono*
1,2,3
,
Mohamed Darwish*
4,5
,
Hideki Uosaki
6,7
,
Nanami Masuyama
8,9,10
,
Motoaki Seki
8,11
,
Hiroyuki Abe
3
,
Nozomu Yachie
8,9,10,12,13
,
Ryohei Yasuda
1
Institution
1
Max Planck Florida Institute for Neuroscience
,
2
Life Science Research Center
,
University of Toyama
,
3
Department of Biochemical Engineering, Graduate School of Science and Engineering
,
Yamagata University
,
4
Graduate School of Innovative Life Science
,
University of Toyama
,
5
Department of Biochemistry, Faculty of Pharmacy
,
Cairo University
,
6
Division of Regenerative Medicine, Center for Molecular Medicine
,
Jichi Medical University
,
7
Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology
,
Jichi Medical University
,
8
Synthetic Biology Division, Research Center for Advanced Science and Technology
,
University of Tokyo
,
9
Institute for Advanced Biosciences
,
Keio University
,
10
Graduate School of Media and Governance
,
Keio University
,
11
Department of Molecular Oncology, Graduate School of Medicine
,
Chiba University
,
12
Department of Biological Sciences, School of Science
,
University of Tokyo
,
13
College of Arts and Sciences
,
University of Tokyo
AUTHORS
Journal (Genetics)
In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
Krzysztof J. Skowronek
1
,
Matthias Bochtler
1
1
International Institute of Molecular and Cell Biology, Warsaw (IIMCB)
Author
Krzysztof J. Skowronek
1
,
Matthias Bochtler
1
Institution
1
International Institute of Molecular and Cell Biology, Warsaw (IIMCB)
AUTHORS
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