JOVE JOURNAL

Biochemistry

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Journal (Biochemistry)

Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs

Russell Spencer-Smith^1,2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Journal (Biochemistry)

Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

Russell Spencer-Smith^1,2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Journal (Biochemistry)

Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions

Héctor Cruz^1,2, Alejandro Llanes^2,3, Patricia L. Fernández^2,3

¹Facultad de Ciencias y Tecnología,Universidad Tecnológica de Panamá (UTP), ²Centro de Biología Molecular y Celular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología AIP (INDICASAT AIP), ³Sistema Nacional de Investigación de Panamá (SNI)

Journal (Biochemistry)

Computational Alanine Scanning Mutagenesis of Protein-Peptide Interface

Héctor Cruz^1,2, Alejandro Llanes^2,3, Patricia L. Fernández^2,3

¹Facultad de Ciencias y Tecnología,Universidad Tecnológica de Panamá (UTP), ²Centro de Biología Molecular y Celular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología AIP (INDICASAT AIP), ³Sistema Nacional de Investigación de Panamá (SNI)

Journal (Biochemistry)

Sequence Diversification of Protein-Peptide Interface with Pepspec

Héctor Cruz^1,2, Alejandro Llanes^2,3, Patricia L. Fernández^2,3

¹Facultad de Ciencias y Tecnología,Universidad Tecnológica de Panamá (UTP), ²Centro de Biología Molecular y Celular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología AIP (INDICASAT AIP), ³Sistema Nacional de Investigación de Panamá (SNI)

Journal (Biochemistry)

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Shreenidhi Rajkumar¹, Danielle Dixon¹, Andrew M. Lipchik², Joshua J. Gruber¹

¹Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences,University of Texas Southwestern Medical Center, ²Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences,Wayne State University

Journal (Biochemistry)

Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation

Shreenidhi Rajkumar¹, Danielle Dixon¹, Andrew M. Lipchik², Joshua J. Gruber¹

¹Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences,University of Texas Southwestern Medical Center, ²Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences,Wayne State University

Journal (Biochemistry)

Measuring Biomolecular Interactions with Weaker Affinities Using Mass Photometry and Microfluidics

Myndert Claasen¹, Zornitsa Kofinova¹, Matteo Contino², Weston B. Struwe^3,4

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry

Myndert Claasen¹, Zornitsa Kofinova¹, Matteo Contino², Weston B. Struwe^3,4

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Analysis of the Microfluidics Coupled Mass Photometry Data to Investigate Low-Affinity Protein-Protein Interactions

Myndert Claasen¹, Zornitsa Kofinova¹, Matteo Contino², Weston B. Struwe^3,4

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Yeast Cell Preparation for FRET Assay for Hyperosmotic Stress Analysis

Constanza Enriquez-Toledo*¹, Cesar A. Ponce-Diego*¹, Cesar L. Cuevas-Velazquez¹

¹Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

Journal (Biochemistry)

Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET

Constanza Enriquez-Toledo*¹, Cesar A. Ponce-Diego*¹, Cesar L. Cuevas-Velazquez¹

¹Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

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