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Imunologia e Infecção
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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

DOI:
10.3791/64918-v

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10:20 min

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March 24, 2023

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, , , , ,
1Quantitative Immuno-hematology, CNRS UMR168,Institut Curie, 2Hamilton Institute,Maynooth University, 3Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, 4Université Paris-Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay

Capítulos

  • 00:04Introduction
  • 00:51Cell Dye Staining
  • 02:33Antibody Staining and Cell Sorting
  • 05:41After Culture Antibody Staining
  • 06:47Post‐Culture Flow Cytometry Data Analysis
  • 08:01Results: Simultaneous Assessment of Cell Division Number, Surface Cell Phenotype, and Cellular Kinship
  • 09:26Conclusion

Summary

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Presented here is a flow cytometry-based technique that allows for simultaneously measuring the number of cell divisions, surface cell phenotype, and cellular kinship. Those properties can be tested statistically using a permutation-based framework.

Tags

Keywords: Hematopoietic Stem CellsHematopoietic Progenitor CellsFlow CytometryCell DivisionCell DifferentiationCFSE LabelingCell FateHigh-throughput AnalysisCell CultureSingle-cell Analysis

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