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Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors
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JoVE Journal Bioquímica
Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

3,873 Views

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07:57 min

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January 20, 2023

DOI:

10.3791/64693-v

DOI:
10.3791/64693-v

•

07:57 min
January 20, 2023

•

47 Views
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1Gangneung Institute of Natural Products,Korea Institute of Science and Technology (KIST), 2School of Life Sciences,University of Sussex

Summary

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The malachite green assay protocol is a simple and cost-effective method to discover heat shock protein 90 (Hsp90) suppressors, as well as other inhibitor compounds against ATP-dependent enzymes.

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Cite this Article

Gupta, S. D., Song, D., Lee, S., Lee, J. W., Park, J., Prodromou, C., Pan, C. Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors. J. Vis. Exp. (191), e64693, doi:10.3791/64693 (2023).

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