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Biochemistry

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JoVE Journal Biochemistry
Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors
 

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

Article DOI: 10.3791/64693-v • 07:57 min • January 20th, 2023
January 20th, 2023 •
1, 1, 1, 1, 1, 2, 1
1Gangneung Institute of Natural Products, Korea Institute of Science and Technology (KIST), 2School of Life Sciences, University of Sussex

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The malachite green assay protocol is a simple and cost-effective method to discover heat shock protein 90 (Hsp90) suppressors, as well as other inhibitor compounds against ATP-dependent enzymes.

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Malachite Green Assay ATPase Proteins ATP Hydrolysis Inorganic Phosphate Detection High-throughput Screening Hsp90 Protein Inhibitors Yeast Hsp90 Measurable ATPase Activity Malachite Green Reagent Optical Density Difference ATP Stock Solution
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