04:30High-Throughput Screening of Hsp90 Inhibitors by the Malachite Green Assay: Assay Design and Addition Orde
06:03Results: Reactions of Malachite Green Reagent, Standard Plot for Standard Phosphate Concentration, and IC50 Calculation for Geldanamycin Against Yeast Hsp90
The malachite green assay protocol is a simple and cost-effective method to discover heat shock protein 90 (Hsp90) suppressors, as well as other inhibitor compounds against ATP-dependent enzymes.