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In vitro Bepaling van plasmodium-geïnfecteerde rode bloedceldoding door cytotoxische lymfocyten
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes
DOI:

08:20 min

August 17, 2022

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Capítulos

  • 00:04Introduction
  • 00:58Experimental Malaria Infection in Mouse
  • 01:49Obtaining Fresh Whole Mouse Splenocytes
  • 03:30Cytotoxic Effector Cell Purification: CD8a+ T Cell Negative Selection
  • 04:22P. yoelii Infected RBC Isolation
  • 05:21CFSE Labeling of RBCs, Preparation for Flow Cytometry and Cytotoxic Lymphocyte Coculture
  • 06:22Results: P. yoelii – iRBC Enrichment and Assessment of cell lysis Percentage of P. yoelii – iRBCs by CD8+ lymphocytes
  • 07:39Conclusion

Summary

Tadução automática

Hier beschrijven we een nieuwe methode om de mechanismen van cellulaire immuniteit tegen Plasmodium tijdens het bloedstadium van infectie op te helderen. Dit is een in vitro test die geïnfecteerde rode bloedcellen dodental door cytotoxische lymfocyten meet.

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