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In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes
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In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes

In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes

1,744 Views

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08:20 min

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August 17, 2022

DOI:

10.3791/63987-v

DOI:
10.3791/63987-v

•

08:20 min
August 17, 2022

•

18 Views
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1Instituto René Rachou,Fundação Oswaldo Cruz, 2Departamento de Bioquímica e Imunologia,Universidade Federal de Minas Gerais

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Here we describe a new method to help elucidate the mechanisms of cellular immunity to Plasmodium during the blood stage of infection. This is an in vitro assay that measures infected red blood cell killing by cytotoxic lymphocytes. 

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In Vitro AssayPlasmodium-infected Red Blood CellsCytotoxic LymphocytesCell-mediated ImmunityMalaria PathogenesisProtective Malaria ImmunitySpleen IsolationRBC LysisMACS Column Purification

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de Lacerda, L., Castro, G., Gomes, C., Junqueira, C. In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes. J. Vis. Exp. (186), e63987, doi:10.3791/63987 (2022).

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