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自由移动小鼠中肠道来源的微生物代谢物的脑室内递送

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

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自由移动小鼠中肠道来源的微生物代谢物的脑室内递送

DOI:

10.3791/63972-v

•

07:49 min

•

June 02, 2022

•

Chia-Wei Liou*^1,2, Tzu-Hsuan Yao*², Wei-Li Wu²

¹Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, ²Department of Physiology, College of Medicine,National Cheng Kung University

Capítulos

00:04Introduction
00:48Stereotaxic Surgery
01:54Commercial Customized Guide Cannula Implantation
03:18Infusion Set Up and System Settings of the Microinjection Controller
05:37Infusion of SCFAs into Lateral Ventricle and Validation of Intracerebroventricular Injection
06:29Results: Analysis of SCFAs-Infused Mice
07:09Conclusion

Summary

Tadução automática

肠道来源的微生物代谢物具有多方面的作用，导致动物的复杂行为。我们的目标是提供一种循序渐进的方法，通过引导套管通过脑室内输送来描述肠道来源的微生物代谢物在大脑中的作用。

Tags

Keywords: Intracerebroventricular Gut Microbiota Gut Metabolites Mouse Brain Comportamento Stereotaxic Guide Cannula N-Butyl Cyanoacrylate Dental Acrylic

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