Journal
/
Neurociência
/
Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

DOI:
10.3791/63972-v

•

07:49 min

•

June 02, 2022

•

, ,
1Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, 2Department of Physiology, College of Medicine,National Cheng Kung University

Capítulos

  • 00:04Introduction
  • 00:48Stereotaxic Surgery
  • 01:54Commercial Customized Guide Cannula Implantation
  • 03:18Infusion Set Up and System Settings of the Microinjection Controller
  • 05:37Infusion of SCFAs into Lateral Ventricle and Validation of Intracerebroventricular Injection
  • 06:29Results: Analysis of SCFAs-Infused Mice
  • 07:09Conclusion

Summary

Tadução automática

Tadução automática

Gut-derived microbial metabolites have multifaceted effects leading to complex behavior in animals. We aim to provide a step-by-step method to delineate the effects of gut-derived microbial metabolites in the brain via intracerebroventricular delivery via a guide cannula.

Tags

Keywords: IntracerebroventricularGut MicrobiotaGut MetabolitesMouse BrainComportamentoStereotaxicGuide CannulaN-Butyl CyanoacrylateDental Acrylic

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/63972?article_type=v

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode
check_url/pt/63972?article_type=v

Recording Single Neurons’ Action Potentials from Freely Moving Pigeons Across Three Stages of Learning
check_url/pt/63972?article_type=v

Recording EEG in Freely Moving Neonatal Rats Using a Novel Method
check_url/pt/63972?article_type=v

Intracerebroventricular Treatment with Resiniferatoxin and Pain Tests in Mice
check_url/pt/63972?article_type=v

Noninvasive EEG Recordings from Freely Moving Piglets
check_url/pt/63972?article_type=v

Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats
check_url/pt/63972?article_type=v

Long-term Sensory Conflict in Freely Behaving Mice
check_url/pt/63972?article_type=v

Hybrid Microdrive System with Recoverable Opto-Silicon Probe and Tetrode for Dual-Site High Density Recording in Freely Moving Mice
check_url/pt/63972?article_type=v

Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals
check_url/pt/63972?article_type=v

Advancements in Multichannel Extracellular Recording for Studying Neuronal Activity in Freely Moving Mice (Video) | JoVE

Read Article