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Hurtig antistof glycoengineering i kinesiske hamster ovarieceller
1Department of Chemical Engineering,Imperial College London, 2Imperial College Centre for Synthetic Biology,Imperial College London, 3BioPharm Process Research,GlaxoSmithKline Research and Development, 4Department of Life Science,Imperial College London, 5School of Chemical & Bioprocess Engineering,University College Dublin
Capítulos
- 00:04Introduction
- 01:00DsiRNA Transfection
- 02:17Buffer Exchange and Sample Concentration
- 03:19Glycan Analysis
- 05:18Results: Confirmation of RNA Interference and Effect of Fut8 Knockdown
- 06:12Conclusion
Glykosyleringsmønsteret for et antistof bestemmer dets kliniske ydeevne, således at industrielle og akademiske bestræbelser på at kontrollere glykosylering vedvarer. Da typiske glykoengineeringskampagner er tids- og arbejdskrævende, ville genereringen af en hurtig protokol til at karakterisere virkningen af glykosyleringsgener ved hjælp af forbigående hæmning vise sig nyttig.
