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Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
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Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
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08:26 min
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April 06, 2022
DOI:
1Center for Theoretical Problems of Physicochemical Pharmacology,Russian Academy of Sciences, 2National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, 3Faculty of Physics,Lomonosov Moscow State University, 4Faculty of Biological and Medical Physics,Moscow Institute of Physics and Technology
Summary
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Here, we describe a set of methods for characterizing the interaction of proteins with membranes of cells or microvesicles.
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Podoplelova, N., Soloveva, P., Garzon Dasgupta, A., Filkova, A., Panteleev, M. Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry. J. Vis. Exp. (182), e63459, doi:10.3791/63459 (2022).
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