/

/

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

JoVE Journal
Biochimica

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biochimica

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

2,174 Views

•

08:26 min

•

April 06, 2022

DOI:

10.3791/63459-v

DOI:

10.3791/63459-v

•

08:26 min

April 06, 2022

•

4 Views

Nadezhda Podoplelova^1,2, Polina Soloveva^1,4, Andrei Garzon Dasgupta^1,2,3, Aleksandra Filkova^1,2, Mikhail Panteleev^1,2,3,4

¹Center for Theoretical Problems of Physicochemical Pharmacology,Russian Academy of Sciences, ²National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, ³Faculty of Physics,Lomonosov Moscow State University, ⁴Faculty of Biological and Medical Physics,Moscow Institute of Physics and Technology

Summary

Here, we describe a set of methods for characterizing the interaction of proteins with membranes of cells or microvesicles.

Explore More Videos

Flow Cytometry Phospholipid Microvesicles Fluorescent-labeled Proteins Kinetic Binding Equilibrium Binding Coagulation Factor X Tyrode’s Buffer FX FD Membrane Interactions Quantitative Analysis

Read Article