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Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
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JoVE Revista Bioquímica
Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

DOI:
10.3791/63459-v

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08:26 min

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April 06, 2022

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, , , ,
1Center for Theoretical Problems of Physicochemical Pharmacology,Russian Academy of Sciences, 2National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, 3Faculty of Physics,Lomonosov Moscow State University, 4Faculty of Biological and Medical Physics,Moscow Institute of Physics and Technology

Capítulos

  • 00:04Introduction
  • 00:58Kinetic Binding Experiments
  • 02:13Equilibrium Binding Experiments
  • 02:45Analysis of Flow Cytometry Data
  • 05:13Converting Fluorescence Intensity to the Mean Number of Binding Sites
  • 07:05Results: Specific Binding of Fluorescent-Labeled Coagulation Factor X or fX to Artificial Phospholipid Vesicles
  • 07:42Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we describe a set of methods for characterizing the interaction of proteins with membranes of cells or microvesicles.

Tags

Flow CytometryPhospholipid MicrovesiclesFluorescent-labeled ProteinsKinetic BindingEquilibrium BindingCoagulation Factor XTyrode’s BufferFX FDMembrane InteractionsQuantitative Analysis

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