Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

L&#233;a Fermont; Nicolas Szydlowski; Christophe Colleoni

doi:10.3791/63392

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Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

JoVE Journal
生化学

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JoVE Journal 生化学

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

2,682 Views

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06:13 min

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March 31, 2022

DOI:

10.3791/63392-v

DOI:

10.3791/63392-v

•

06:13 min

March 31, 2022

•

7 Views

Léa Fermont¹, Nicolas Szydlowski^1,2, Christophe Colleoni¹

¹CNRS, UMR8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle,University of Lille, ²CNRS, USR3290-MSAP-Miniaturisation pour la Synthèse, l’Analyse et la Protéomique,University of Lille

概要

In the present protocol, the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) technique is used to determine the chain length distribution (CLD) and the average chain length (ACL) of glycogen.

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Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

概要

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