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定量微管分离技术分离小鼠组织中稳定的微管、不稳定的微管和游离微管蛋白

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

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定量微管分离技术分离小鼠组织中稳定的微管、不稳定的微管和游离微管蛋白

DOI:

10.3791/63358-v

•

07:21 min

•

November 17, 2023

•

Ayaka Hagita-Tatsumoto², Tomohiro Miyasaka^2,3

¹Department of Neuropathology, Faculty of Life and Medical Sciences,Doshisha University, ²Center for Research in Neurodegenerative Diseases,Doshisha University, ³Laboratory of Physiology and Anatomy, School of Pharmacy,Nihon University

Capítulos

00:05Introduction
00:49Isolation and Homogenization of Mouse Tissues to Quantify Microtubule Fractions
01:53Fractionation of Mouse Tissues
04:04Results: Analysis of the Characterized Murine Microtubule Brain Fractions
06:25Conclusion

Summary

Tadução automática

微管是微管蛋白聚合物，在真核细胞中作为细胞骨架成分起着至关重要的作用，并以其动态不稳定性而闻名。本研究开发了一种将微管分离为稳定微管、不稳定微管和游离微管蛋白的方法，以评估微管在各种小鼠组织中的稳定性。

Tags

Keywords Extracted From The Given Text: Quantitative Microtubule Fractionation Stable Microtubules Labile Microtubules Free Tubulin Microtubule Stability Alzheimer’s Disease Cancer Phosphate Buffer Solution PBS Microtubule Stabilizing Buffer MSB Centrifugation Supernatant Precipitate Sodium Dodecyl Sulfate SDS Sample Buffer Western Blotting

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