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Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
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Bioquímica
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JoVE Journal Bioquímica
Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

1,529 Views

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07:21 min

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November 17, 2023

DOI:

10.3791/63358-v

DOI:
10.3791/63358-v

•

07:21 min
November 17, 2023

•

3 Views
,
1Department of Neuropathology, Faculty of Life and Medical Sciences,Doshisha University, 2Center for Research in Neurodegenerative Diseases,Doshisha University, 3Laboratory of Physiology and Anatomy, School of Pharmacy,Nihon University

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Keywords Extracted From The Given Text:Quantitative Microtubule FractionationStable MicrotubulesLabile MicrotubulesFree TubulinMicrotubule StabilityAlzheimer’s DiseaseCancerPhosphate Buffer SolutionPBSMicrotubule Stabilizing BufferMSBCentrifugationSupernatantPrecipitateSodium Dodecyl SulfateSDS Sample BufferWestern Blotting

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Cite this Article

Hagita-Tatsumoto, A., Miyasaka, T. Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues. J. Vis. Exp. (201), e63358, doi:10.3791/63358 (2023).

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