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流式细胞术分析鉴定鼠肺先天免疫细胞和适应性免疫细胞
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
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流式细胞术分析鉴定鼠肺先天免疫细胞和适应性免疫细胞

DOI:
10.3791/62985-v

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09:57 min

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November 16, 2021

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, , , ,
1Division of Hematology-Oncology,Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine,Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College

Capítulos

  • 00:04Introduction
  • 01:17Surgical Excision and Tissue Preparation
  • 02:11Preparation of Single-Cell Suspension
  • 04:12Surface Antibody Staining
  • 05:25Cell Fixation and Intracellular Staining
  • 06:46Results: Gating Strategy
  • 09:10Conclusion

Summary

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Tadução automática

在这项研究中，我们提出了一种有效且可重复的方案来分离小鼠呼吸系统的免疫群体。我们还提供了一种方法，使用基于9色的流式细胞术面板来鉴定驻留在健康小鼠肺部的所有先天性和适应性免疫细胞。

Tags

Lung Immune CellsMyeloid CellsNon-myeloid CellsFlow CytometryGating StrategyLung Immune Landscape

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