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Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

English

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5,568 Views

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09:57 min

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November 16, 2021

DOI:

10.3791/62985-v

DOI:
10.3791/62985-v

•

09:57 min
November 16, 2021

•

16 Views
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1Division of Hematology-Oncology,Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine,Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College

Summary

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In this study, we present an effective and reproducible protocol to isolate the immune populations of the murine respiratory system. We also provide a method for the identification of all innate and adaptive immune cells that reside in the lungs of healthy mice, using a 9-color-based flow cytometry panel.

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Cite this Article

Christofides, A., Cao, C., Pal, R., Aksoylar, H. I., Boussiotis, V. A. Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung. J. Vis. Exp. (177), e62985, doi:10.3791/62985 (2021).

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