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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
 

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Article DOI: 10.3791/62985-v • 09:57 min • November 16th, 2021
November 16th, 2021 •
1,2, 1,2,3, 1,2, 1,2, 1,2
1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College

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In this study, we present an effective and reproducible protocol to isolate the immune populations of the murine respiratory system. We also provide a method for the identification of all innate and adaptive immune cells that reside in the lungs of healthy mice, using a 9-color-based flow cytometry panel.

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Flow Cytometric Analysis Identification Innate Immune Cells Adaptive Immune Cells Murine Lung Gating Strategies Comparison Of Results Comprehensive And Reproducible Gating Strategy Pulmonary Myeloid Immune Populations Non-myeloid Immune Populations Nine Markers Characterization Steady-state Conditions Disease Models Lung Immune Landscape Digestion Conditions Reagents Release Of Immune Cells Euthanized Animal Preparation Surgery Preparation Skin Removal Lung Flushing
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