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检测蛋白质低洼构象状态的高压NMR实验
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Bioquímica
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JoVE Journal Bioquímica
High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
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检测蛋白质低洼构象状态的高压NMR实验

DOI:
10.3791/62701-v

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04:37 min

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June 29, 2021

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, ,
1Department of Chemistry,Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology,Iowa State University

Capítulos

  • 00:05Introduction
  • 00:34Sample Preparation and High-Pressure Cell Assembly
  • 02:03Recording High-Pressure NMR Experiments
  • 02:32Results: Unfolding of hnRNPA1 RRM2 Domain Under Pressure
  • 04:11Conclusion

Summary

Tadução automática

Tadução automática

我们详细描述了组装高压电池所需的步骤，建立并记录了高压NMR实验，最后分析了压力下的峰值强度和化学移位变化。这些实验可以为蛋白质的折叠途径和结构稳定性提供有价值的见解。

Tags

Keywords: High-pressure NMRProtein Conformational StatesPressure PerturbationGlass CavitiesVoid VolumesNitrogen-15-labeled SampleMineral OilTransmission LiquidPressure Cell AssemblyTransverse Relaxation Optimized SpectroscopyHetero Single-quantum Coherence SpectroscopyFolding/unfolding RatesRRM2Heterogeneous Nuclear Ribonucleoprotein A1

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