/
/
High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
2,489 Views
•
04:37 min
•
June 29, 2021
DOI:
Summary
Automatically generated
We provide a detailed description of the steps required to assemble a high-pressure cell, set up and record high-pressure NMR experiments, and finally analyze both peak intensity and chemical shift changes under pressure. These experiments can provide valuable insights into the folding pathways and structural stability of proteins.
Explore More Videos
High-pressure NMRProtein Conformational StatesPressure PerturbationGlass CavitiesVoid VolumesNitrogen-15-labeled SampleMineral OilTransmission LiquidPressure Cell AssemblyTransverse Relaxation Optimized SpectroscopyHetero Single-quantum Coherence SpectroscopyFolding/unfolding RatesRRM2Heterogeneous Nuclear Ribonucleoprotein A1
Read Article
Cite this Article
Nguyen, T. T., Siang, S., Roche, J. High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States. J. Vis. Exp. (172), e62701, doi:10.3791/62701 (2021).
Copy