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High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
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High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

2,459 Views

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04:37 min

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June 29, 2021

DOI:

10.3791/62701-v

DOI:
10.3791/62701-v

•

04:37 min
June 29, 2021

•

1 Views
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1Department of Chemistry,Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology,Iowa State University

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High-pressure NMRProtein Conformational StatesPressure PerturbationGlass CavitiesVoid VolumesNitrogen-15-labeled SampleMineral OilTransmission LiquidPressure Cell AssemblyTransverse Relaxation Optimized SpectroscopyHetero Single-quantum Coherence SpectroscopyFolding/unfolding RatesRRM2Heterogeneous Nuclear Ribonucleoprotein A1

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Nguyen, T. T., Siang, S., Roche, J. High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States. J. Vis. Exp. (172), e62701, doi:10.3791/62701 (2021).

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