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High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

2,489 Views

04:37 min

June 29, 2021

DOI:

04:37 min
June 29, 2021

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Summary

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We provide a detailed description of the steps required to assemble a high-pressure cell, set up and record high-pressure NMR experiments, and finally analyze both peak intensity and chemical shift changes under pressure. These experiments can provide valuable insights into the folding pathways and structural stability of proteins.

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