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High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
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High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
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04:37 min
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June 29, 2021
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We provide a detailed description of the steps required to assemble a high-pressure cell, set up and record high-pressure NMR experiments, and finally analyze both peak intensity and chemical shift changes under pressure. These experiments can provide valuable insights into the folding pathways and structural stability of proteins.
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Nguyen, T. T., Siang, S., Roche, J. High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States. J. Vis. Exp. (172), e62701, doi:10.3791/62701 (2021).
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