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使用下一代测序来鉴定与CD4启动子附近CAS9诱导的双链断裂修复相关的突变
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JoVE Journal Genética
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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使用下一代测序来鉴定与CD4启动子附近CAS9诱导的双链断裂修复相关的突变

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06:59 min

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March 31, 2022

DOI:

10.3791/62583-v

DOI:
10.3791/62583-v

•

06:59 min
March 31, 2022

•

3 Views
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1Division of Biology,Kansas State University, 2Kansas State Veterinary Diagnostic Laboratory,Kansas State University, 3Department of Diagnostic Medicine/Pathobiology,Kansas State University

Summary

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这里介绍的是sgRNA / CAS9内切酶和下一代测序方案，可用于鉴定与CD4启动子附近的双链断裂修复相关的突变。

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Cite this Article

Hu, C., Doerksen, T., Bugbee, T., Wallace, N. A., Palinski, R. Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter. J. Vis. Exp. (181), e62583, doi:10.3791/62583 (2022).

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