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隔离和成像现场， 通过颤音分割老鼠肾佩尔维斯的精致起搏器区域
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning
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隔离和成像现场， 通过颤音分割老鼠肾佩尔维斯的精致起搏器区域

DOI:
10.3791/62040-v

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07:38 min

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April 30, 2021

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, ,
1Department of Physiology & Cell Biology,University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology,University of California School of Medicine, 3Department of Life & Health Sciences,Dundalk Institute of Technology

Capítulos

  • 00:05Introduction
  • 00:44Preparation and Dissection of Kidney and Calibration of Vibratome Instrument
  • 02:32Vibratome Sectioning and Kidney Slice Ca2+ Image Acquisition
  • 04:06Kidney Slice Ca2+ Image Acquisition
  • 05:14Results: Imaging of Spontaneous Calcium Ion Transient in Vibratome Sections of Whole Kidneys
  • 07:01Conclusion

Summary

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Tadução automática

此协议的目标是使用振动切除隔离小鼠肾骨盆的完整心脏起搏器区域。然后，这些部分可用于原位 Ca2+ 成像，以阐明心脏起搏器细胞和振动切片中其他间质细胞的 Ca2+ 瞬态特性。

Tags

Pacemaker RegionsRenal PelvisVibratome SectioningIntact PreparationSmooth MuscleKidneyUreterCalcium Ion ImagingDissectionRazor BladeKRB Solution

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