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Modellazione in vitro per malattie neurologiche utilizzando la conversione diretta dai fibroblasti alle cellule progenitrici neuronali e la differenziazione in astrociti
JoVE Journal
Neurociência
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JoVE Journal Neurociência
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
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Modellazione in vitro per malattie neurologiche utilizzando la conversione diretta dai fibroblasti alle cellule progenitrici neuronali e la differenziazione in astrociti

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11:42 min

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June 10, 2021

DOI:

10.3791/62016-v

DOI:
10.3791/62016-v

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11:42 min
June 10, 2021

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7 Views
, , , , , ,
1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2College of Medicine, The Ohio State University

Summary

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Descriviamo un protocollo per riprogrammare i fibroblasti derivati dalla pelle umana in cellule progenitrici neuronali indotte (INPC) e la loro successiva differenziazione in Astrociti indotti (iA). Questo metodo porta a una generazione rapida e riproducibile di iNPC e iA in grandi quantità.

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Cite this Article

Dennys, C. N., Sierra-Delgado, J. A., Ray, S. S., Hartlaub, A. M., Roussel, F. S., Rodriguez, Y., Meyer, K. In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes. J. Vis. Exp. (172), e62016, doi:10.3791/62016 (2021).

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