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Transpupillær to-foton In vivo Billeddannelse af musens nethinde
1John F. Hardesty, MD Department of Ophthalmology and Visual Sciences,Washington University School of Medicine, 2Graduate Program in Neuroscience,Washington University School of Medicine, 3Department of Neuroscience,Washington University School of Medicine, 4Hope Center for Neurological Disorders,Washington University School of Medicine
Capítulos
- 00:05Introduction
- 00:42Imaging Preparation
- 03:44Two-Photon Image Acquisition
- 06:13Mouse Recovery
- 06:46Results: Representative In Vivo Mouse Retina Imaging
- 08:36Conclusion
In vivo billeddannelse er et kraftfuldt værktøj til undersøgelse af biologi i sundhed og sygdom. Denne protokol beskriver transpupillær billeddannelse af musens nethinde med et standard to-fotonmikroskop. Det demonstrerer også forskellige in vivoimaging metoder til fluorescerende mærkning af flere cellulære kohorter i nethinden.
