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Preparação da amostra TMT para envio de instalações proteômicas e análise de dados subsequentes

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Preparação da amostra TMT para envio de instalações proteômicas e análise de dados subsequentes

11,970 Views

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07:44 min

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June 08, 2020

DOI:

10.3791/60970-v

DOI:

10.3791/60970-v

•

07:44 min

June 08, 2020

•

8 Views

Silveli Suzuki-Hatano, Ang-Chen Tsai, Audrey Daugherty, Christina A. Pacak

¹Department of Pediatrics,University of Florida

Tags

TMT Sample Preparation Proteomics Protein Extraction Labeling Efficiency Reproducibility Data Acquisition CHAPS Lysis Buffer Bead Disruptor Sonication Reducing/alkylating Reagent Treatment TCEP Iodoacetamide Methanol/chloroform Precipitation

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