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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

11,970 Views

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07:44 min

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June 08, 2020

DOI:

10.3791/60970-v

DOI:
10.3791/60970-v

•

07:44 min
June 08, 2020

•

8 Views
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1Department of Pediatrics,University of Florida

Tags

TMT Sample PreparationProteomicsProtein ExtractionLabeling EfficiencyReproducibilityData AcquisitionCHAPS Lysis BufferBead DisruptorSonicationReducing/alkylating Reagent TreatmentTCEPIodoacetamideMethanol/chloroform Precipitation

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Suzuki-Hatano, S., Tsai, A., Daugherty, A., Pacak, C. A. TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis. J. Vis. Exp. (160), e60970, doi:10.3791/60970 (2020).

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