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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
JoVE Journal
Biochemie
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JoVE Journal Biochemie
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

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07:44 min

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June 08, 2020

DOI:

10.3791/60970-v

DOI:
10.3791/60970-v

•

07:44 min
June 08, 2020

•

10 Views
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1Department of Pediatrics,University of Florida

Summary

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We present an optimized tandem mass tag (TMT) labeling protocol that includes detailed information for each of the following steps: protein extraction, quantification, precipitation, digestion, labeling, submission to a proteomics facility, and data analyses.

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Suzuki-Hatano, S., Tsai, A., Daugherty, A., Pacak, C. A. TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis. J. Vis. Exp. (160), e60970, doi:10.3791/60970 (2020).

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