Journal
/
Bioquímica
/
蛋白质组学设施提交和后续数据分析的TMT样品制备
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Please note that all translations are automatically generated. Click here for the English version.

蛋白质组学设施提交和后续数据分析的TMT样品制备

12,053 Views

•

07:44 min

•

June 08, 2020

DOI:

10.3791/60970-v

DOI:
10.3791/60970-v

•

07:44 min
June 08, 2020

•

10 Views
, , ,
1Department of Pediatrics,University of Florida

Summary

Automatically generated

我们提出了优化的串联质量标签 （TMT） 标签协议，其中包括以下每个步骤的详细信息：蛋白质提取、定量、沉淀、消化、标记、提交到蛋白质组学设施以及数据分析。

Explore More Videos

TMT Sample PreparationProteomicsProtein ExtractionLabeling EfficiencyReproducibilityData AcquisitionCHAPS Lysis BufferBead DisruptorSonicationReducing/alkylating Reagent TreatmentTCEPIodoacetamideMethanol/chloroform Precipitation

Read Article

Cite this Article

Suzuki-Hatano, S., Tsai, A., Daugherty, A., Pacak, C. A. TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis. J. Vis. Exp. (160), e60970, doi:10.3791/60970 (2020).

Download .ris file

Copy

Share Video

.

Copy