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大鼠异位心脏和心肌细胞移植的免疫模型
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats
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大鼠异位心脏和心肌细胞移植的免疫模型

DOI:
10.3791/60956-v

•

09:25 min

•

May 08, 2020

•

, , , , ,
1Regenerative Medicine and Experimental Surgery, Department of General, Visceral and Transplant Surgery,Hannover Medical School

Capítulos

  • 00:04Introduction
  • 00:53Heart Explantation and Perfusion
  • 03:13Heart Implantation
  • 06:24Enzymatic Heart Digestion and Subcutaneous Cardiac Cell Injection
  • 08:08Results: Representative Analyses of the Specificity of the Immune Response in Heterotopic Heart Transplant Rejection
  • 09:00Conclusion

Summary

Tadução automática

Tadução automática

我们描述了大鼠异位腹心移植的模型，这意味着对当前策略的修改，从而导致简化的手术方法。此外，我们还描述了一种新的排斥模型，通过注射重要的心肌细胞的耳朵内，允许进一步的移植免疫学分析在大鼠。

Tags

RatHeterotopic Heart TransplantationCardiac Muscle Cell TransplantationSurgical ModelImmunological ResearchDonor Heart ExplantationHeparinExsanguinationVascular Ligation

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