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Immunology and Infection

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JoVE Journal Immunology and Infection
An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats
 

An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats

Article DOI: 10.3791/60956-v • 09:25 min • May 8th, 2020
May 8th, 2020 •
1, 1, 1, 1, 1, 1
1Regenerative Medicine and Experimental Surgery, Department of General, Visceral and Transplant Surgery, Hannover Medical School

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We describe a model of heterotopic abdominal heart transplantation in rats, implying modifications of current strategies, which lead to a simplified surgical approach. Additionally, we describe a novel rejection model by in-ear injection of vital cardiac muscle cells, allowing further transplant immunological analyses in rats.

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Immunological Model Heterotopic Heart Transplantation Cardiac Muscle Cell Transplantation Rats Surgical Expertise Feasible Approach Crucial Steps Tolerance Rejection Teaching Model Surgical Training Donor Heart Explantation Anesthetized Rat Abdominal Fur Removal Thoracic Fur Removal Supine Position Heating Base Elastic Bands Disinfect Skin Median Laparotomy Retractors Mobilize Intestine Inferior Vena Cava Puncture Intravenous Injection
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