Journal
/
Neurociência
/
激光捕获胶质瘤分区域的微切，用于肿瘤内异质性、肿瘤流和入侵的空间和分子表征
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
Please note that all translations are automatically generated. Click here for the English version.

激光捕获胶质瘤分区域的微切，用于肿瘤内异质性、肿瘤流和入侵的空间和分子表征

DOI:
10.3791/60939-v

•

09:09 min

•

April 12, 2020

•

, , , , , ,
1Dept. of Neurosurgery,University of Michigan Medical School, 2Dept. of Cell and Developmental Biology,University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science,University of Michigan Medical School, 4Rogel Cancer Center,University of Michigan Medical School

Capítulos

  • 00:05Introduction
  • 01:17Animal Perfusion and Brain Preservation
  • 02:01Glioma Brain Tumors Tissue Cryopreservation
  • 02:52Frozen Brain Tumor Tissue Sectioning
  • 03:58Cryopreserved Brain Tissue Section Fixation and Staining
  • 04:48Laser Capture Microdissection
  • 06:30Results: Representative RNA Quality Control and Library Preparation
  • 07:55Conclusion

Summary

Tadução automática

Tadução automática

激光微解剖（LMD）是一种敏感且高度可重复的技术，可用于揭示介导胶质瘤异质性和入侵的途径。在这里，我们描述了一个优化的协议，使用激光LMD从胶质瘤组织中分离离散区域，然后进行转录分析。

Tags

Laser MicrodissectionRNA SequencingIntratumor HeterogeneityMesenchymal CellsTumor InvasionTumor MorphologyMolecular ProfilesTumor ProgressionTherapeutic TargetsBioluminescencePerfusionCryopreservationSpatial CharacterizationMolecular Characterization

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

激光捕获显微切割技术的果蝇外周神经元

总RNA的分离和下游下一代测序和基因表达谱的非激光捕获显微切割离散小鼠脑地区显微镜方法

激光捕获显微切割的丰富种群的创伤性脑损伤后神经元或单个神经元的基因表达分析

神经元的分化的人类Neuroprogenitor细胞培养激光捕获显微切割

通过激光捕获微分路，从小鼠脊髓运动神经细胞体中生成RNA进行转录分析

通过鼻腔活检获得联合激光捕获显微切割的嗅觉神经元：一种可能的方法来研究治疗反应的精神障碍

激光捕获显微切割 - 个人多巴胺神经元的分离和整个腹侧被盖区的示范

立体定向激光捕获创伤性脑区显微解剖

激光捕获显微切割分离新鲜冷冻人体肠神经节的优化

一种从人类死后小脑中的激光捕获微解剖 purkinje 细胞中分离高质量 rna 的不锈钢协议

Read Article