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小鼠胚胎细胞的多路复用单细胞mRNA测序分析
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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小鼠胚胎细胞的多路复用单细胞mRNA测序分析

12,215 Views

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08:30 min

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January 07, 2020

DOI:

10.3791/60647-v

DOI:
10.3791/60647-v

•

08:30 min
January 07, 2020

•

7 Views
, ,
1Department of Developmental Biology,University of Pittsburgh School of Medicine

Summary

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在这里，我们提出了一种多路复用单细胞mRNA测序方法，用于分析小鼠胚胎组织中的基因表达。基于滴滴的单细胞mRNA测序（scRNA-Seq）方法与多路复用策略相结合，可以同时分析来自多个样品的单个细胞，从而显著降低试剂成本并最大限度地减少实验批次效应。

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Cite this Article

Feng, W., Przysinda, A., Li, G. Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells. J. Vis. Exp. (155), e60647, doi:10.3791/60647 (2020).

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