/
/
Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
This content is Free Access.
Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
9,369 Views
•
08:43 min
•
January 31, 2020
DOI:
Summary
Automatically generated
This protocol provides detailed experimental steps to establish a three-dimensional in vitro culture of bladder tumor organoids derived from carcinogen-induced murine bladder cancer. Culture methods including passaging, genetic engineering, and orthotopic transplantation of tumor organoids are described.
Read Article
Cite this Article
Kim, Y., Lee, J., Kim, S., Shin, K. Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids. J. Vis. Exp. (155), e60469, doi:10.3791/60469 (2020).
Copy