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Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
Chapters
- 00:04Title
- 00:50In Vitro Culture of Bladder Tumor Organoids
- 03:50Genetic Manipulation of Bladder Organoids via Lentivirus-mediated Transduction
- 04:36Orthotopic Transplantation of Bladder Organoids
- 07:06Results: Culture and Transplantation of Bladder Tumor Organoids
- 08:01Conclusion
This protocol provides detailed experimental steps to establish a three-dimensional in vitro culture of bladder tumor organoids derived from carcinogen-induced murine bladder cancer. Culture methods including passaging, genetic engineering, and orthotopic transplantation of tumor organoids are described.
