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Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
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Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
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January 31, 2020
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This protocol provides detailed experimental steps to establish a three-dimensional in vitro culture of bladder tumor organoids derived from carcinogen-induced murine bladder cancer. Culture methods including passaging, genetic engineering, and orthotopic transplantation of tumor organoids are described.
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Kim, Y., Lee, J., Kim, S., Shin, K. Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids. J. Vis. Exp. (155), e60469, doi:10.3791/60469 (2020).
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