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Stereologisk vurdering af kolinerge fiberlængde i Nucleus Basalis af Meynert af Mouse Brain
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1Laboratory for Alzheimer’s Disease and Aging Research,Kansas City Veterans Affairs Medical Center, 2Department of Neurology,University of Kansas Medical Center, 3Department of Molecular and Integrative Physiology,University of Kansas Medical Center, 4The University of Kansas Alzheimer’s Disease Center
Capítulos
- 00:04Introduction
- 01:09Perfusion and Tissue Processing
- 02:45Systematic Section Selection
- 03:19Immunohistochemistry (IHC)
- 04:46Stereology
- 08:10Results: Representative Mouse Brain Cholinergic Fiber Length Estimation
- 08:38Conclusion
Neuronal fiber længde inden for en tre-dimensionel struktur i en hjerne region er en pålidelig parameter til at kvantificere specifikke neuronal strukturel integritet eller degeneration. Denne artikel beskriver en stereologisk kvantificeringsmetode til måling af kolinerge fiberlængde i meynerts kernebasalis i mus som et eksempel.
Tags
Keywords:
Stereological EstimationCholinergic Fiber LengthNucleus Basalis Of MeynertMouse BrainNeurodegenerationNeuronal Fiber LossCell Body LossStereological QuantificationUnbiased FractionationLine-like StructuresNeuronal FibersTranscardial PerfusionDPBSPFASucroseCryotomeSectioningImmunohistochemical LabelingNormal Bovine SerumTriton X-100Tris-buffered Saline
