需要订阅 JoVE 才能查看此. 登录或开始免费试用。
1Laboratory for Alzheimer’s Disease and Aging Research,Kansas City Veterans Affairs Medical Center, 2Department of Neurology,University of Kansas Medical Center, 3Department of Molecular and Integrative Physiology,University of Kansas Medical Center, 4The University of Kansas Alzheimer’s Disease Center
Chapters
- 00:04Introduction
- 01:09Perfusion and Tissue Processing
- 02:45Systematic Section Selection
- 03:19Immunohistochemistry (IHC)
- 04:46Stereology
- 08:10Results: Representative Mouse Brain Cholinergic Fiber Length Estimation
- 08:38Conclusion
Neuronal fiber length within a three-dimensional structure of a brain region is a reliable parameter to quantify specific neuronal structural integrity or degeneration. This article details a stereological quantification method to measure cholinergic fiber length within the nucleus basalis of Meynert in mice as an example.
Tags
Keywords:
Stereological EstimationCholinergic Fiber LengthNucleus Basalis Of MeynertMouse BrainNeurodegenerationNeuronal Fiber LossCell Body LossStereological QuantificationUnbiased FractionationLine-like StructuresNeuronal FibersTranscardial PerfusionDPBSPFASucroseCryotomeSectioningImmunohistochemical LabelingNormal Bovine SerumTriton X-100Tris-buffered Saline
