/
/
Stereological Estimation of Cholinergic Fiber Length in the Nucleus Basalis of Meynert of the Mouse Brain
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Stereological Estimation of Cholinergic Fiber Length in the Nucleus Basalis of Meynert of the Mouse Brain
5,323 Views
•
09:15 min
•
February 05, 2020
DOI:
1Laboratory for Alzheimer’s Disease and Aging Research,Kansas City Veterans Affairs Medical Center, 2Department of Neurology,University of Kansas Medical Center, 3Department of Molecular and Integrative Physiology,University of Kansas Medical Center, 4The University of Kansas Alzheimer’s Disease Center
Summary
Automatically generated
Neuronal fiber length within a three-dimensional structure of a brain region is a reliable parameter to quantify specific neuronal structural integrity or degeneration. This article details a stereological quantification method to measure cholinergic fiber length within the nucleus basalis of Meynert in mice as an example.
Explore More Videos
Stereological EstimationCholinergic Fiber LengthNucleus Basalis Of MeynertMouse BrainNeurodegenerationNeuronal Fiber LossCell Body LossStereological QuantificationUnbiased FractionationLine-like StructuresNeuronal FibersTranscardial PerfusionDPBSPFASucroseCryotomeSectioningImmunohistochemical LabelingNormal Bovine SerumTriton X-100Tris-buffered Saline
Read Article
Cite this Article
Singh, P., Peng, D. W., Suo, W. Z. Stereological Estimation of Cholinergic Fiber Length in the Nucleus Basalis of Meynert of the Mouse Brain. J. Vis. Exp. (156), e60405, doi:10.3791/60405 (2020).
Copy