Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Francesco Simone Ruggeri; Tomas &#352;neideris; Sean Chia; Michele Vendruscolo; Tuomas  P. J. Knowles

doi:10.3791/60108

Journal

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Bioquímica

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Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

9,479 Views

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12:58 min

•

September 12, 2019

DOI:

10.3791/60108-v

DOI:

10.3791/60108-v

•

12:58 min

September 12, 2019

•

5 Views

Francesco Simone Ruggeri¹, Tomas Šneideris^1,2, Sean Chia¹, Michele Vendruscolo¹, Tuomas P. J. Knowles^1,3

¹Centre for Misfolding Diseases, Department of Chemistry,University of Cambridge, ²Institute of Biotechnology, Life Sciences Center,Vilnius University, ³Cavendish Laboratory, Department of Physics,University of Cambridge

Summary

We describe the application of infrared nanospectroscopy and high-resolution atomic force microscopy to visualize the process of protein self-assembly into oligomeric aggregates and amyloid fibrils, which is closely associated with the onset and development of a wide range of human neurodegenerative disorders.

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Protein Aggregates Infrared Nanospectroscopy Atomic Force Microscopy Single Molecule Methods Biomolecular Processes Nano Scale Heterogeneous Systems Protein Aggregation Biomaterials Drug Delivery AFM Parameters AFM Imaging AFM Cantilever AFM Laser Beam AFM Deflected Beam AFM Oscillation AFM Resonance

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Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

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