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Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
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Biochimica
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JoVE Journal Biochimica
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

DOI:
10.3791/60108-v

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12:58 min

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September 12, 2019

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, , , ,
1Centre for Misfolding Diseases, Department of Chemistry,University of Cambridge, 2Institute of Biotechnology, Life Sciences Center,Vilnius University, 3Cavendish Laboratory, Department of Physics,University of Cambridge

Capitoli

  • 00:04Titolo
  • 01:09Atomic Force Microscopy (AFM) Protein Aggregate Morphology Imaging
  • 05:09Infrared (IR) Nanospectroscopy Protein Aggregate Measurement
  • 11:57Results: Individual Protein Aggregated Characterization by IR Nanospectroscopy and AFM
  • 13:30Conclusion

Summary

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We describe the application of infrared nanospectroscopy and high-resolution atomic force microscopy to visualize the process of protein self-assembly into oligomeric aggregates and amyloid fibrils, which is closely associated with the onset and development of a wide range of human neurodegenerative disorders.

Tags

Protein AggregatesInfrared NanospectroscopyAtomic Force MicroscopySingle Molecule MethodsBiomolecular ProcessesNano ScaleHeterogeneous SystemsProtein AggregationBiomaterialsDrug DeliveryAFM ParametersAFM ImagingAFM CantileverAFM Laser BeamAFM Deflected BeamAFM OscillationAFM Resonance

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