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Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
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Biochimica
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JoVE Journal Biochimica
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

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12:58 min

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September 12, 2019

DOI:

10.3791/60108-v

DOI:
10.3791/60108-v

•

12:58 min
September 12, 2019

•

5 Views
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1Centre for Misfolding Diseases, Department of Chemistry,University of Cambridge, 2Institute of Biotechnology, Life Sciences Center,Vilnius University, 3Cavendish Laboratory, Department of Physics,University of Cambridge

Summary

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We describe the application of infrared nanospectroscopy and high-resolution atomic force microscopy to visualize the process of protein self-assembly into oligomeric aggregates and amyloid fibrils, which is closely associated with the onset and development of a wide range of human neurodegenerative disorders.

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Ruggeri, F. S., Šneideris, T., Chia, S., Vendruscolo, M., Knowles, T. P. J. Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy. J. Vis. Exp. (151), e60108, doi:10.3791/60108 (2019).

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