/
/
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
8,517 Views
•
08:04 min
•
October 08, 2019
DOI:
Summary
Automatically generated
Protein binding microarray (PBM) experiments combined with biochemical assays link the binding and catalytic properties of DNA primase, an enzyme that synthesizes RNA primers on template DNA. This method, designated as high-throughput primase profiling (HTPP), can be used to reveal DNA-binding patterns of a variety of enzymes.
Read Article
Cite this Article
Ilic, S., Cohen, S., Afek, A., Gordan, R., Lukatsky, D. B., Akabayov, B. DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling. J. Vis. Exp. (152), e59737, doi:10.3791/59737 (2019).
Copy