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CRISPR-Cas9를 사용하여 꿀벌 뇌 섹션에서 항 RDL 및 안티 mGlutR1 수용체 항체 테스트
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9
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CRISPR-Cas9를 사용하여 꿀벌 뇌 섹션에서 항 RDL 및 안티 mGlutR1 수용체 항체 테스트

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09:25 min

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January 30, 2020

DOI:

10.3791/59993-v

DOI:
10.3791/59993-v

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09:25 min
January 30, 2020

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, , , , , , , ,
1Department of Neuroscience,University of Arizona, 2School of Life Sciences,Arizona State University, 3Department of Scientific and Technologic Investigations,University of Sonora, 4Integrated DNA Technologies, Inc.

Tags

CRISPR-Cas9Gene EditingHoneybee BrainIonotropic GABAA ReceptorsMetabotropic Glutamate ReceptorsRDLMGlutR1Immunocytochemical AnalysisGuide RNARibonucleoprotein ComplexCas9 Nuclease

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Sinakevitch, I., Kurtzman, Z., Choi, H. G., Ruiz Pardo, D. A., Dahan, R. A., Klein, N., Bugarija, B., Wendlandt, E., Smith, B. H. Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9. J. Vis. Exp. (155), e59993, doi:10.3791/59993 (2020).

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