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在免疫缺陷小鼠中表演人类骨骼肌异种移植物

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice

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在免疫缺陷小鼠中表演人类骨骼肌异种移植物

DOI:

10.3791/59966-v

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07:48 min

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September 16, 2019

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Kyla A. Britson², Aaron D. Black³, Kathryn R. Wagner^2,3,4, Thomas E. Lloyd^2,4

¹Graduate Program in Cellular and Molecular Medicine,Johns Hopkins University School of Medicine, ²Department of Neurology,Johns Hopkins University School of Medicine, ³Kennedy Krieger Institute, ⁴Department of Neuroscience,Johns Hopkins University School of Medicine

Capítulos

00:04Título
00:35Surgical Preparation
01:48Xenograft Surgery
03:52Xenograft Harvest
05:43Results: Representative Human Skeletal Muscle Xenograft Analyses
07:12Conclusion

Summary

Tadução automática

在传统的实验室模型系统中，复杂的人类疾病对建模可能具有挑战性。在这里，我们描述了一种通过将人类骨骼肌活检移植到免疫缺陷小鼠中来模拟人类肌肉疾病的外科方法。

Tags

Human Skeletal Muscle Xenografts Immunodeficient Mice Muscle Disease Modeling Preclinical Therapeutic Testing Human Muscle Cell Biology Rare Or Acquired Muscle Diseases Muscle Biopsy NOD-Rag-gamma Mouse Tibialis Anterior Extensor Digitorum Longus Peroneus Longus Surgical Procedure

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