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Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

13,879 Views

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06:55 min

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July 17, 2019

DOI:

10.3791/59833-v

DOI:
10.3791/59833-v

•

06:55 min
July 17, 2019

•

19 Views
, , ,
1Department of Psychiatry and Behavioral Sciences,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine

Summary

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Generating differentiated cell types from human pluripotent stem cells (hPSCs) holds great therapeutic promise but remains challenging. PSCs often exhibit an inherent inability to differentiate even when stimulated with a proper set of signals. Described here is a simple tool to enhance multilineage differentiation across a variety of PSC lines.

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Cite this Article

Sambo, D., Li, J., Brickler, T., Chetty, S. Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation. J. Vis. Exp. (149), e59833, doi:10.3791/59833 (2019).

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