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Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
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発生生物学
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JoVE Journal 発生生物学
Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

13,879 Views

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06:55 min

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July 17, 2019

DOI:

10.3791/59833-v

DOI:
10.3791/59833-v

•

06:55 min
July 17, 2019

•

19 Views
, , ,
1Department of Psychiatry and Behavioral Sciences,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine

概要

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Generating differentiated cell types from human pluripotent stem cells (hPSCs) holds great therapeutic promise but remains challenging. PSCs often exhibit an inherent inability to differentiate even when stimulated with a proper set of signals. Described here is a simple tool to enhance multilineage differentiation across a variety of PSC lines.

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Cite this Article

Sambo, D., Li, J., Brickler, T., Chetty, S. Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation. J. Vis. Exp. (149), e59833, doi:10.3791/59833 (2019).

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