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הערכה של אינטראקציות חלבונים-חלבונים באמצעות טכניקת העיכול האנטי-ממברנה
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Bioquímica
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JoVE Journal Bioquímica
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
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הערכה של אינטראקציות חלבונים-חלבונים באמצעות טכניקת העיכול האנטי-ממברנה

6,367 Views

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07:07 min

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July 19, 2019

DOI:

10.3791/59733-v

DOI:
10.3791/59733-v

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07:07 min
July 19, 2019

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6 Views
, , , ,
1Division of Biological Chemistry, Department of Molecular Biology,Showa University School of Pharmacy, 2Department of Biochemistry,Showa University School of Medicine

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Protein-protein InteractionOn-membrane DigestionProteomic AnalysisImmunoprecipitationMagnetic BeadsPVDF MembraneMass Spectrometry

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Obama, T., Miyazaki, T., Aiuchi, T., Miyazaki, A., Itabe, H. Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique. J. Vis. Exp. (149), e59733, doi:10.3791/59733 (2019).

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